Orihuela Diaz Pedro Alejandro

Email: 
pedro.orihuela@usach.cl
Teléfono: 
27 181 119
Sitio web: 
http://www.experts.scival.com/UdeSantiago/expert.asp?n=PEDRO%20A%20ORIHUELA%20DI…
Research

Our research is involved to study the physiology of the female genital tract at cellular and molecular level. We are mainly focused to elucidate the genomic and non-genomic signaling pathways of estradiol or 2-methoxyestradiol that regulate reproductive events associated with egg transport, sperm migration or embryo development. Our aim is to find new molecular markers of estrogens to design new therapies that discriminate between genomic and non-genomic effects of estradiol and its metabolites.

Línea(s) de investigación: 
Fisiología Reproductiva
Jerarquía: 
Asociado
Grados académicos /educación: 
Doctor en Ciencias Biológicas, P. Universidad Católica de Chile, 2002
Biólogo, Universidad Nacional Mayor de San Marcos, Lima, Perú, 1992
Publicaciones: 
  1. Parada-Bustamante A, Valencia C, Reuquén P, Rincón-Rodriguez R, Orihuela PA. Role of 2-methoxyestradiol, and endogenous estrogen metabolite, in health and disease. Mini-Reviews in Medicinal Chemistry 2015; 15: 427-438.
    Reuquén P, Oróstica ML, Rojas I, Díaz P, Parada-Bustamante A, Orihuela PA. Reproduction (2015); 150:331-341.
  2. Rabagliati FM, Zapata PA, Orihuela PA, Galvez P, Canales D, Cardenas H. Preparation and characterization of a new polymer/pharmaceutical-based composite. Part I: Meloxicam. Polymer Bulletin (2014); 71: 3323-3331.
  3. Oróstica ML, López J, Zuñiga LM, Utz D, Díaz P, Reuquén P, Parada-Bustamante A, Cardenas A, Orihuela PA. Mating decreases plasma levels of TGF1 and regulates myosalpinx expression of TGF1/TGFBR3 in the rat. Molecular Reproduction & Development (2014); 81: 1053-1061.
  4. Oróstica ML, Lopez J, Rojas I, Rocco J, Díaz P, Reuquén P, Cardenas H, Parada-Bustamante A, Orihuela PA. Estradiol increases cAMP in the oviductal secretory cells through a nongenomic mechanism. Reproduction (2014); 148: 285-294.
  5. Rincón-Rodriguez RJ, Oróstica ML, Díaz P, Reuquén P, Cárdenas H, Orihuela PA. Changes in the expression pattern induced by 2-methoxyestradiol in the mouse uterus. Endocrine (2013); 44: 773-783.
  6. Orostica ML, Zuñiga LM, Utz D, Parada-Bustamante A, Velasquez LA, Cardenas H, Orihuela PA. Tumor necrosis factor-alpha is the signal induced by mating to shutdown a 2-methoxyestradiol nongenomic action necessary to accelerate  viductal egg transport in the rat. Reproduction. (2013); 145: 109-117.
  7. Parada-Bustamante A, Croxatto HB, Cárdenas H, Orihuela PA. Differential participation of endothelin receptors in estradiol-induced oviductal egg transport acceleration in unmated and mated rats. Asian Pacific Journal of Reproduction (2012); 1: 22-26.
  8. Díaz PS, Solar PA, Juica NE, Orihuela PA, Cardenas H, Christodoulides M, Vargas R, Velasquez LA. Differential expression of extracelular matrix components in the Fallopian tube throughout the menstrual cycle. Reproductive Biology & Endocrinology (2012); 10: 56.
  9. O. Saavedra-Dahm, P. Solar, H. Díaz, A. Mandel, M. Casado, PA. Orihuela, M. Rivera, L. Velásquez, H. Cardenas. The Case Against Template Informed Consent Procedures in Biomedical Research: Heterogeneity in Health Literacy in Chile. Terapia Psicológica. (2012); 30: 127-131.
  10. Velasquez L, García K, Morales F, Heckels JE, Orihuela PA, Rodas PI, Christodoulides M, Cardenas H. Neisseria gonorrhoeae pilus attenuates cytokine response of human fallopian tube explants. J Biomed Biotechnol. (2012); 2012: 491298.
  11. Ríos M, Parada-Bustamante A, Velásquez LA, Croxatto HB, Orihuela PA. Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats. Reprod Biol Endocrinol. (2011); 9: 69-79.
  12. Garcia KP, Rubilar PS, Vargas MF, Cárdenas H, Rios M, Orihuela PA, Vargas RH, Fuher J, Heckels JE, Christodoulides M, Velasquez LA. Nitric oxid is not involved in Neisseria gonorroeae-induced cellular damage of human Falopian tubes in vitro. Biological Research, (2010); 43: 39-50
  13. Parada-Bustamante A, Orihuela PA, Rios M, Cuevas CA, Oróstica ML, Velasquez LA, Villalón MJ, Croxatto HB. A non-genomic signaling pathway shut down by mating changes the estradiol-induced gene expression profile in the rat oviduct. Reproduction (2010); 139: 631-644.
  14. Orihuela PA, Ulipristal: a progesterone receptor antagonist as an emergency contraceptive. Experts Reviews in Obstetrics & Gynecology (2010); 5: 13-17.
Proyectos: 
  • “The role of the extracelular matrix-associated proteins during in vitro fallopian tube infection by Neisseria gonorrhoeae”. FONDECYT 1120712. Co-investigador (2012-2015).
  • “Anillo vaginal no hormonal que inhibe la ovulación” CORFO 12IDL2. Director Alterno (2012-2014).
  • “Signaling Cascades Elucidated by TNF-a to shut down an estradiol non-genomic pathway in oviductal epithelial cells”. FONDECYT 1110662. Investigador Responsable (2011-2014).
  • “Centro De Desarrollo En Nanociencia Y Nanotecnología-CEDENNA”. Programa De Financiamiento Basal Para Centros Científicos Y Tecnológicos De Excelencia. Investigador Asociado (2009-2014).
  • “Cambios Celulares y Moleculares de la matriz extracelular durante el ciclo menstrual y su papel en la infección in Vitro de explantes de Trompa de Falopio con Neisseria gonorroheae” FONDECYT 1090589. Co-investigador (2009-2012).
  • “Mecanismos Celulares y Moleculares Activados por el coito Implicados en el Silenciamiento de la Vía de Señalización No-Genómica del Estradiol en el Oviducto” FONDECYT 1080523. Investigador Responsable (2008-2011).